Flaxseed (Linum usitatissimum) Extract Activity on Human Oral Fibroblasts (HOrF) Cell Line

Abstract

Introduction: General health benefits have been demonstrated by natural products. Flaxseed (Linum usitatissimum) extract’s bioactivities have been reported in many studies such as antimicrobial, anti-oxidant, and anti-inflammatory effects. In addition, flaxseed extract has skin wound healing activity and potential for treating oral ulcers. Methodology: L. usitatissimum was extracted using 70% ethanol via soxhlet method, gas chromatography mass spectrum (GC-MS) was used to illustrate the components of L. usitatissimum extract. Human oral fibroblasts (HOrF) were cultured to be used for testing the activity of L. usitatissimum. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to assess the HOrF cells viability at different time point; 24, 48 and 72 hours. Wound healing assay was used to monitor the healing of HOrF cells after treating them with flaxseed extract. The results of the assay were obtained at 18, 24, 48 and 72 hours. Results: The results of GC-MS illustrate the major components present in L. usitatissimum extract. The effect of L. usitatissimum extract on HOrF cells show high proliferation effect at 24 and 48 hours while the highest proliferation effect was recorded at 72 hours’ post-treatment. Wound healing assay results show healing activity of L. usitatissimum extract on HOrF cells as fast as 18 hours’ post-treatment. Conclusion: L. usitatissimum extract have proliferating and healing effects on HOrF cell line. Therefore, it can be considered as a potential promising oral wound healing agent.