Technical Improvement in DNA Preparation for Pulsedâ€â€Field Gel Electrophoresis

Authors

  • Thong Kwai Lin
  • Lai Kin Seng
  • Goh Yee Ling

Abstract

We report a rapid pulsed-field electrophoresis (PFGE) protocol for subtyping $Salmonella$ isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results obtained using this modified, rapid protocol were highly reproducible.

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Published

28-08-2003

Issue

Vol. 22 No. 2 (2003)

Section

Original Articles

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How to Cite

Technical Improvement in DNA Preparation for Pulsed—Field Gel Electrophoresis. (2003). Malaysian Journal of Science (MJS), 22(2), 55-59. https://mjir.um.edu.my/index.php/MJS/article/view/8516

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