Dental Anomalies and Muscle Segment Homeobox1 Gene Polymorphism in Non-syndromic Cleft Lip with or without Palate Children
DOI:
https://doi.org/10.22452/adum.vol30.4Keywords:
Cleft lip , Cleft palate, Hypodontia, Mutation, Polymerase chain reactionAbstract
This study aims to determine the prevalence of dental anomalies and MSX1 gene 799G>T polymorphism and its association with non-syndromic cleft lip with or without palate (NSCL±P) attending Hospital Universiti Sains Malaysia. Clinical and radiological assessments on 37 NSCL±P patients and 80 non-cleft children were done to detect dental anomalies. The buccal cells were collected and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used to identify polymorphism. NSCL±P was higher among males (54%) and mostly unilateral cleft lip and palate (51.3%). The prevalence of dental anomalies in morphology in NSCL±P was 18.9% (95% CI: 5.7%, 32.2%) and non-cleft was 6.3% (95% CI: 0.8%, 11.7%). Hypodontia in NSCL±P was 75% (95% CI: 61.2%, 90.2%) and non-cleft was 7.5% (95% CI: 1.6%, 13.4%). There was a significant association between NSCL±P and anomalies in morphology (P= 0.04; OR=3.5)) and number (P< 0.01; OR= 40). There was an absence of rare 799G>T polymorphism in all NSCL±P and non-cleft children indicating that all samples contain common 799G polymorphism. In conclusion, the prevalence of dental anomalies in morphology and number was significantly higher in NSCL±P compared to non-cleft children. However, it was not significantly associated with MSX1 799G>T polymorphism.
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Mohammd Shah NS, Sulong S, Wan Sulaiman WA, Halim AS. Two novel genes TOX3 and COL21A1 in large extended Malay families with nonsyndromic cleft lip and /palate. Molecular genetic and Genomic Medicine. 2019; 7(5):e635
Kathariya MD, Nikam AP, Chopra K, Patil NN, Raheja H, Kathariya R. Prevalence of dental anomalies among school going children in India. Journal of International Oral Health. 2013; 5(5):10-14.
Camporesi, M., Baccetti, T., Marinelli, A., Defraia, E., & Franchi, L. Maxillary dental anomalies in children with cleft lip and palate: a controlled study. International Journal of Paediatric Dentistry. 2010; 20(6):442–450
Al-Kharboush GH, Al-Balkhi KM, Al-Moammar K. The prevalence of specific dental anomalies in a group of Saudi cleft lip and palate patients. Saudi Medical Journal. 2015; 27(2):75–80.
Phan M., Conte F, Khandelwal KD, Ockeloen CW, Bartzela T, Kleefstra T et.al Tooth agenesis and orofacial clefting: genetic brothers in arms? Human genetic 2016; 135(12):1299-327
Dixon MJ, Marazita ML, Beaty TH, Murray JC. Cleft lip and palate: understanding genetic and environmental influences. Nature Reviews Genetics. 2011; 12(3):167-178.
Singh VP, Ramu D. Association of MSX1 799 G> T variant with nonsyndromic cleft lip/palate in South Indian adolescent patients. International Journal of Paediatric Dentistry. 2012; 22(3):228-231.
Paradowska-Stolarz A, Dubowik M, Szeląg J, Kawala B. Dental anomalies in the incisor-canine region in patients with cleft lip and palate-literature review. Developmental Period Medicine. 2014; 18(1):66-69.
Mossey PA, Little J, Munger RG, Dixon MJ, Shaw WC. Cleft lip and palate. The Lancet. 2009; 374(9703):1773-1785.
Bartzela T, Theuerkauf B, Reichart E, Speimann Opitz. Clinical characterization of 266 patients and family members with cleft and /or palate with associated malformation and syndromes. Clinical Oral Investigations. 2021; 25(9):5531-5540
Akcam MO, Evirgen S, Uslu O, Memikoğlu UT. Dental anomalies in individuals with cleft lip and/or palate. European Journal of Orthodontics. 2010; 32(2):207-213.
Raducanu AM, Didilescu AC, Feraru IV, Dumitrache MA, Hantoiu TA, IonescuI E. Considerations on morphological abnormalities of permanent teeth in children with cleft lip and palate. Romanion Journal of Morphology and Embryology. 2015; 56(2):453.
Yordanova-Krostova GR, Grancharov MV, Gurgurovo GD. Abnormality in morphogenesis of tooth development and relationship with orthodontic deformities and treatment approaches. Case report in Dentistry. 2021; 1183504.
da Silva APRB, Costa B, de Carvalho, Carrara CF. Dental anomalies of number in the permanent dentition of patients with bilateral cleft lip: radiographic study. Cleft Palate Craniofacial Journal. 2008; 45(5):473-476.
Al Jamal GA, Hazza'a AM, Rawashdeh Ma. A Prevalence of dental anomalies in a population of cleft lip and palate patients. The Cleft Palate-Craniofacial Journal. 2010; 47(4):413-420.
Hua F, He H, Ngsn P, Bouzid W. Prevalence of peg-shaped maxillary permanent lateral incisors: A meta- analysis. American Journal of Orthodontics and Dentofacial Orthopedics. 2013; 144(1): 97-109.
Chanchala H, Nandlal B. Coexistent peg shaped mandibular central incisors along with maxillary lateral incisors-A rare case. International Journal of Oral and Maxillofacial Pathology. 2012; 3(1):65-68.
Wong H, Lai M, King N. Dental anomalies in Chinese children with cleft lip and palate. Dentistry. 2:127.
Miletich I, Sharpe PT. Normal and abnormal dental development. Human Molecular Genetics. 12(suppl 1), R69-R73. 2003.
Aizenbud D, Camasuvi S, Peled M, Brin I. Congenitally missing teeth in the Israeli cleft population. The Cleft Palate-Craniofacial Journal. 2005; 42(3):314-317.
Tereza GPG, de Carvalho CCF, Costa B. Tooth abnormalities of number and position in the permanent dentition of patients with complete bilateral cleft lip and palate. The Cleft Palate-Craniofacial Journal. 2010; 47(3):247-252.
Pemberton TJ, Das P, Patel PI. Hypodontia: genetics and future perspectives. Brazilian Journal Oral Sciences. 2005; 4(13):695-706.
Lekkas C, Latief B, Ter Rahe S, Kuijpers-Jagtman A. The adult unoperated cleft patient: absence of maxillary teeth outside the cleft area. The Cleft Palate-Craniofacial Journal. 2000; 37(1):17-20.
Lai, MC, King NM, Wong HM. Abnormalities of maxillary anterior teeth in Chinese children with cleft lip and palate. The Cleft Palate-Craniofacial Journal. 2009; 46(1): 58-64.
Lehtonen V, Anttonen V, Ylikontiola L, Koskinen S, Pesonen P, Sándor G. Dental anomalies associated with cleft lip and palate in Northern Finland. European Journal of Paediatric Dentistry. 2015; 16(4):327-332.
Jezewski P, Vieira A, Nishimura C, Ludwig B, Johnson M, O’brien S, et al. Complete sequencing shows a role for MSX1 in non-syndromic cleft lip and palate. Journal of Medical Genetics. 2003; 40(6):399-407.
Suwal P, Ayer A, Roy DK, Singh VP. MSX1 Polymorphism in an Eastern Nepalese Non Syndromic cleft lip/palate patient population. International Journal of Interdisciplinary and Multidisciplinary Studies. 2014; 1(8):149-151.
Jyothish A, Geroge A, Narayanan PV, Krishnamurthy RG. MSX1 Gene Plymorphisms in patients with Non-syndromic Cleft lip and palate: A Tertiary Care Centre Based Case Control Study from Central Kerala. The Cleft Palate Craniofacial Journal, 2023;15: 10556656231214131
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